FIG. 10.

Electron micrographs of Vero cells infected with rHSV-RYC after prefixation at 24 h p.i. followed by freezing and freeze-substitution. (A) Low-power micrograph showing Golgi membranes, virions within a vacuole (arrow), and capsids (arrowheads) within the cytoplasm and budding at Golgi membranes, respectively. (B) Virion within the perinuclear space of the nuclear envelope. (C) Accumulation of capsids in a crystalline manner within the nucleus. (D) Budding capsid at Golgi membranes. (E) Virion within a concentric vacuole. (F) Virions in the extracellular space. Bars, 500 nm (A and C) and 100 nm (B, D, and E).