FIG. 5.

Phenotype of rAd5, rAd26, and rAd48 vectors in rhesus monkeys. Cytokine secretion phenotypes of CD8⁺ (A, C, E) and CD4⁺ (B, D, F) T lymphocytes elicited by rAd5-Gag (A, B), rAd26-Gag (C, D), and rAd48-Gag (E, F) vectors in the study described for Fig. 3 were determined by multiparameter flow cytometry at week 4 following immunization. Gag-specific absolute (bar graphs) and proportional (pie charts) IFN-γ, TNF-α, and IL-2 responses in all combinations are depicted in CD8⁺ and CD4⁺ CM and EM T-lymphocyte subpopulations. Mean responses with standard errors are shown. +, present; −, absent.