TABLE 1.
Virus replication assaya
| Virus | Genotype | Cell type infected/cell type used for plaque assay | Titer (PFU/ml) |
|---|---|---|---|
| vJB11 | Deletion of codons 143 to 151 of UL26.5 | Vero/CV26.5b | No viral plaques detected |
| vJB11 | Deletion of codons 143 to 151 of UL26.5 | CV26.5/CV26.5 | 8 × 105 |
| vJB11 | Deletion of codons 143 to 151 of UL26.5 | CV26c/CV26.5 | 6 × 105 |
| vJB11 | Deletion of codons 143 to 151 of UL26.5 | CV26Md/CV26.5 | No plaques detected |
| vJB11 | Deletion of codons 143 to 151 of UL26.5 | CV26.5Me/CV26.5 | No plaques detected |
| vJB11R | Deletion repaired | Vero/Vero | 2.5 × 107 |
| vJB11R | Deletion repaired | CV26.5/Vero | 3 × 107 |
| HSV-1(F) | Wild type | CV26.5/Vero | 2 × 107 |
| HSV-1(F) | Wild type | CV26.5M/Vero | 2.4 × 107 |
| HSV-1(F) | Wild type | CV26M/Vero | 2 × 107 |
a
Cells were infected with the indicated viruses at 0.1 PFU/cell. Twenty-four hours after infection, virus was harvested by three cycles of freezing and thawing, and infectious yields were determined by plaque assay on the indicated cell monolayers.
b
CV26.5 cells constitutively express UL26.5.
c
CV26 cells constitutively express UL26.
d
CV26M cells constitutively express UL26 lacking codons 449 to 457.
e
CV26.5M cells constitutively express UL26.5 lacking codons 143 to 151.