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. 2008 Mar 5;82(10):4774–4784. doi: 10.1128/JVI.02651-07

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FIG. 5. — Physical interaction between M-MOK and CcO1. (A) Yeast two-hybrid analysis of the CcO1 interaction with M-MOK and M-THA. β-Galactosidase activity of Y187::CG1945 S. cerevisiae transformed with the indicated bait (AD) and prey (BD) plasmids is revealed by an X-Gal overlay assay. An empty vector (E) is used as a control. (B) CcO1-matrix complexes were immunoprecipitated with anti-CcO1 antibody from transfected HeLa cells expressing M-MOK or M-THA fused to 3×Flag and immunoblotted with anti-CcO1 and anti-Flag antibodies. (Top) Input lysates containing M-MOK and M-THA, as well as their corresponding immunoprecipitation (IP-CcO1) complexes after treatment with anti-CcO1 antibody. Bands corresponding to antibody heavy and light chains are defined by asterisks. (Bottom) Duplicate of the same blot in the top panel that was developed after treatment with anti-Flag antibody.