FIG. 6.

M-MOK and fragments M1 and M1.2 inhibit CcO activity. (A) CcO activity was evaluated in noninfected HeLa cells (NI) as well as HeLa cells infected with THA and MOK by spectrocolorimetry at 24 h and 48 h p.i. The measurements were performed after protein normalization and were expressed as the percentages of activity relative to that for NI. All values were obtained from three independent experiments. (B) HeLa cells were transfected with plasmids expressing EGFP alone, full-length M fused to EGFP (M-MOK), or its truncated forms (M1, M1.1, and M1.2). CcO activity was evaluated 24 h posttransfection (p.t.). The measurements were performed after protein normalization and were expressed as the percentages of activity relative to CcO activity in cells expressing EGFP alone. All values were obtained from three independent experiments. Significant effects are indicated by asterisks, pound signs, and plus signs (P < 0.05). (C) Immunoblot experiment with anti-EGFP showing expression of different protein constructs at 24 h p.t. Bands are obtained from samples with equal protein content and a single exposure time.