FIG. 3.
Phenotype and function of E2445-HKFNSSGCPERL-456-specific CD8+ T cells through the course of a homologous HCV rechallenge in chimpanzee CB0572. (A) Flow cytometric analysis of PBMCs at various time points after repeat inoculation as indicated above plots. Plots are gated as described in the legend for Fig. 1, and percentages indicated in plots are as per the legend for Fig. 1. Quadrant gates are derived from appropriate fluorescence minus one controls. (B) Intracellular cytokine staining for IFN-γ production by E2445-specific CD8+ T cells 3 weeks post-homologous viral rechallenge. Plots show surface expression of CD69 versus intracellular staining for IFN-γ following 6 h in vitro incubation at 37°C in the presence of brefeldin A, without peptide (top panel) versus incubation with E2445-457 peptide (bottom panel). Plots are gated on forward and side scatter appropriate for lymphocytes and CD8+ Patr-B*2301/E2445-457-positive cells. Percentages indicate the proportion of CD8+ Patr-B*2301/E2445-457-positive cells in each quadrant.