FIG. 4.

Simultaneous detection of S-phase cells and p130 in organotypic cultures expressing E7 with gain- or loss-of-function mutations in the CKII recognition sequence or substrates. PHKs were acutely transduced with a retrovirus expressing the indicated E7 mutation. BrdU was added to the media for the last 12 h before harvest. p130 (green) and BrdU (red) incorporation are shown for substitution mutations in HPV-18 E7 (A, B, and C), HPV-11 E7 (D), or HPV-1 E7 (E). Left panels show p130 (green), middle panels show BrdU (red) plus DAPI, and right panels show merged images. All sections were imaged at ×20 magnification.