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. Author manuscript; available in PMC: 2009 Apr 15.
Published in final edited form as: Immunol Lett. 2007 Dec 26;117(1):35–44. doi: 10.1016/j.imlet.2007.11.017

Fig. 4. Transient interference with the expression of CTCF mRNA reverses the HIV-1 resistant phenotype in HRF(+) cells.

Fig. 4

HRF(+) and HRF(-) cells were transfected by CTCF or control Litmus siRNA fragments then challenged with NL4-3 HIV-1 at concentration of 0.5pg/cell. Replication of HIV-1 was tested by RT-PCR of the single-spliced vif transcript (A); detection of the p24 core antigen (B); biological activity of cell culture supernatants was tested by RSA (C). Data presented in this figure show one of the three separate experiments.