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. 1997 Oct 14;94(21):11381–11386. doi: 10.1073/pnas.94.21.11381

Figure 6.

Figure 6

Effect of wortmannin. Cultures were treated with dimethyl sulfoxide vehicle (□) or 100 nM wortmannin (░⃞) at 37°C for 0.5 hr, followed by treatment with insulin or IGF-I for 1.5 hr. Then, cells were briefly trypsinized, and recovery of surface GH binding was measured 2 hr after trypsinization. Vs. dimethyl sulfoxide control, ∗, P < 0.05 and ‡, P < 0.01. vs. dimethyl sulfoxide insulin; †, P < 0.05. vs. wortmannin control; ¶, P = 0.01.