TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or feature(s) | Reference or source |
|---|---|---|
| Strains | ||
| P. methylutens DM12R | Rifr derivative of wild-type strain DM12; contains plasmids pMTH1 (32 kb), pMTH4 (22 kb), pMTH2 (approximately 200 kb), and pMTH3 (greater than 650 kb) | 2 |
| P. pantotrophus KL100 | Rifr derivative of wild-type strain DSM 11073; deprived of indigenous plasmid pKLW1 | 2 |
| E. coli DH5Δlac | deoR thi1 relA1 supE44 endA1 gyrA96 recA1 hsdR17Δ(argF lac)U169 Nalr | M. Yarmolinsky |
| E. coli TG1 | supE hsdΔ5 thi Δ(lac proAB), F′ (traD36 proAB+lacIqlacZΔM15) | Laboratory collection |
| Plasmids | ||
| pMEC1 | Kmr; mobilizable shuttle (E. coli-Paracoccus spp.) trap plasmid carrying cI-tetA selection cartridge | 4 |
| pDS132 | Cmr; mobilizable cloning vector; contains sacB gene; oriT RK2 | 18 |
| pBBR1MCS-3 | Tcr; ori pBBR1; cloning broad-host-range vector; oriT RK2 | 13 |
| pEBB10 | Tcr; pBBR1MCS-3 carrying sacB | This study |
| pABW1 | Kmr; ori pMB1; mobilizable cloning vector; oriT RK2 | 3 |
| pABW12 | pABW1 carrying a 0.7-kb HindIII fragment of pMTH4 | This study |
| pMTH4::pABW12 | Cointegrate of pMTH4 and pABW12 | This study |
| pCM132 | Kmr; ori RK2; lacZ reporter gene fusion vector | 14 |
| pCM132TC | pCM132 carrying promoterless tetA | This study |
| pRS551 | Kmr; ori pMB1; lacZ reporter gene fusion vector | 22 |
| pRK2013 | Kmr; helper plasmid carrying genes for conjugal transfer of RK2 | 9 |