Strains |
|
|
P. methylutens DM12R |
Rifr derivative of wild-type strain DM12; contains plasmids pMTH1 (32 kb), pMTH4 (22 kb), pMTH2 (approximately 200 kb), and pMTH3 (greater than 650 kb) |
2 |
P. pantotrophus KL100 |
Rifr derivative of wild-type strain DSM 11073; deprived of indigenous plasmid pKLW1 |
2 |
E. coli DH5Δlac
|
deoR thi1 relA1 supE44 endA1 gyrA96 recA1 hsdR17Δ(argF lac)U169 Nalr
|
M. Yarmolinsky |
E. coli TG1 |
supE hsdΔ5 thi Δ(lac proAB), F′ (traD36 proAB+lacIqlacZΔM15) |
Laboratory collection |
Plasmids |
|
|
pMEC1 |
Kmr; mobilizable shuttle (E. coli-Paracoccus spp.) trap plasmid carrying cI-tetA selection cartridge |
4 |
pDS132 |
Cmr; mobilizable cloning vector; contains sacB gene; oriT RK2 |
18 |
pBBR1MCS-3 |
Tcr; ori pBBR1; cloning broad-host-range vector; oriT RK2 |
13 |
pEBB10 |
Tcr; pBBR1MCS-3 carrying sacB
|
This study |
pABW1 |
Kmr; ori pMB1; mobilizable cloning vector; oriT RK2 |
3 |
pABW12 |
pABW1 carrying a 0.7-kb HindIII fragment of pMTH4 |
This study |
pMTH4::pABW12 |
Cointegrate of pMTH4 and pABW12 |
This study |
pCM132 |
Kmr; ori RK2; lacZ reporter gene fusion vector |
14 |
pCM132TC |
pCM132 carrying promoterless tetA
|
This study |
pRS551 |
Kmr; ori pMB1; lacZ reporter gene fusion vector |
22 |
pRK2013 |
Kmr; helper plasmid carrying genes for conjugal transfer of RK2 |
9 |