TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| P. damselae subsp. piscicida | ||
| PC554.2 | Virulent strain; prfC::ICEPdaSpa1 Tcr | 24 |
| DI21 | Virulent strain | 28 |
| CS31 | DI21 derivative; Rfr Knr | 32 |
| E. coli | ||
| DH5α | Cloning strain | Laboratory stock |
| XL1-Blue MR | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac | Stratagene |
| MC1061 | F−araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (Smr) hsdR2(rK− mK+) mcrA mcrB1 | 36 |
| CAG18420 | MG1655 lacZU118 lacI42::Tn10kan | 34 |
| BI533 | MG1655 Nxr | 21 |
| BW25113 | lacIqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBAADLD78 | 16 |
| VI499 | BW25113 derivative; recA | |
| CCW026 | ICEPdaSpa1 exconjugant of MC1061; Smr Tcr | This study |
| CCW01 | BW25113 prfC::ICEPdaSpa1 | This study |
| CCW02 | BW25113 prfC::ICEPdaSpa1 Δint | This study |
| CCW03 | VI499 prfC::ICEPdaSpa1 | This study |
| CCW069 | MC1061 transformed with pPHDP10-Cm; Smr Cmr | This study |
| CCW077 | CCW026 transformed with pPHDP10-Cm; Smr Tcr Cmr | This study |
| CCW088 | Nxr Tcr Cmr exconjugant from CCW077 × BI533 | This study |
| CCW129 | Tcr Cmr Apr exconjugant from PC554.2 × VI499 transformed with pPHDP10-Cm and with pGG2B; recA | This study |
| Plasmids | ||
| pSuperCos1 | Cosmid cloning vector; Apr Knr | Stratagene |
| pPHDP10 | Native virulence plasmid of P. damselae subsp. piscicida DI21 strain | 17 |
| pPHDP10-Cm | pPHDP10 with a Cmr cassette from pKD3 inserted in BamHI site; Cmr | This study |
| pKD3 | PCR template for one-step chromosomal gene inactivation; Cmr | 16 |
| pGG2B | setCD genes from SXTMO10 cloned into pBAD30; Apr | V. Burrus |