Strains |
|
|
P. damselae subsp. piscicida
|
|
|
PC554.2 |
Virulent strain; prfC::ICEPdaSpa1 Tcr
|
24 |
DI21 |
Virulent strain |
28 |
CS31 |
DI21 derivative; Rfr Knr
|
32 |
E. coli
|
|
|
DH5α |
Cloning strain |
Laboratory stock |
XL1-Blue MR |
Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac
|
Stratagene |
MC1061 |
F−araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (Smr) hsdR2(rK− mK+) mcrA mcrB1
|
36 |
CAG18420 |
MG1655 lacZU118 lacI42::Tn10kan
|
34 |
BI533 |
MG1655 Nxr
|
21 |
BW25113 |
lacIqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBAADLD78
|
16 |
VI499 |
BW25113 derivative; recA
|
|
CCW026 |
ICEPdaSpa1 exconjugant of MC1061; Smr Tcr
|
This study |
CCW01 |
BW25113 prfC::ICEPdaSpa1 |
This study |
CCW02 |
BW25113 prfC::ICEPdaSpa1 Δint
|
This study |
CCW03 |
VI499 prfC::ICEPdaSpa1 |
This study |
CCW069 |
MC1061 transformed with pPHDP10-Cm; Smr Cmr
|
This study |
CCW077 |
CCW026 transformed with pPHDP10-Cm; Smr Tcr Cmr
|
This study |
CCW088 |
Nxr Tcr Cmr exconjugant from CCW077 × BI533 |
This study |
CCW129 |
Tcr Cmr Apr exconjugant from PC554.2 × VI499 transformed with pPHDP10-Cm and with pGG2B; recA
|
This study |
Plasmids |
|
|
pSuperCos1 |
Cosmid cloning vector; Apr Knr
|
Stratagene |
pPHDP10 |
Native virulence plasmid of P. damselae subsp. piscicida DI21 strain |
17 |
pPHDP10-Cm |
pPHDP10 with a Cmr cassette from pKD3 inserted in BamHI site; Cmr
|
This study |
pKD3 |
PCR template for one-step chromosomal gene inactivation; Cmr
|
16 |
pGG2B |
setCD genes from SXTMO10 cloned into pBAD30; Apr
|
V. Burrus |