TABLE 2.
Genome region and predicted ORF or product (no. of amino acids) | Positions of ORF in ICEPdaSpa1a | Gene or protein homologue | GenBank accession no. for homologue gene | % Identity/% similarity to homologueb |
---|---|---|---|---|
rumB region | ||||
TetA (400) | 11676-12878 (compl) | P. damselae subsp. piscicida tetracycline resistance protein, class D | BAA03719 | 66/78 |
TetR (200) | 12959-13561 | E. coli Tet repressor, class D | 1A6I | 74/85 |
TnpI (96) | 13951-14241 | Psychromonas ingrahamii IS3/IS911 transposase | ZP_01350213 | 62/80 |
TnpII (243) | 14373-15104 | Psychromonas ingrahamii integrase | ZP_01350212 | 64/80 |
Region between s035′ and ′s035 | ||||
IS10 transposase (402) | 28188-29396 (compl) | Salmonella enterica serovar Typhi IS10 transposase | NP_058298 | 100/100 |
Hot spot 2 | ||||
Spa01 (909) | 52041-54770 | Psychromonas sp. strain CNPT3 putative helicase | ZP_01215992 | 40/60 |
Spa02 (369) | 54767-55876 | No database matches with significant similarity | ||
Spa03 (510) | 55889-57421 | Acidovorax sp. strain JS42 phospholipase D/transphosphatidylase | ZP_01383961 | 28/47 |
Spa04 (377) | 57736-58869 | Desulfovibrio desulfuricans two-component transcriptional regulator, Fis family | ABB37621 | 38/61 |
Hot spot 4 | ||||
Spa05 (175) | 68950-69474 (compl) | No database matches with significant similarity | ||
Spa06 (257) | 69487-70257 (compl) | Salmonella enterica hypothetical protein | AAG03008 | 38/52 |
Hot spot 3 | ||||
IS10 transposase (402) identical to the IS10 transposase listed above | 81904-83112 (compl) | Salmonella enterica serovar Typhi IS10 transposase | NP_058298 | 100/100 |
Spa07 (831) | 83835-86330 | Deltaproteobacterium MLMS-1 heat shock protein Hsp70 | EAT04212 | 80/88 |
Spa08 (489) | 86334-87803 | Deltaproteobacterium MLMS-1 AAA ATPase, central region | EAT04211 | 82/91 |
Spa09 (553) | 87816-89477 | Deltaproteobacterium MLMS-1 hypothetical protein | EAT04215 | 68/82 |
Spa10 (235) | 89470-90177 | No database matches with significant similarity | ||
Spa11 (464) | 90286-91680 | Deltaproteobacterium MLMS-1 peptidoglycan-binding domain 1 | EAT04214 | 39/51 |
ψvrlR | 91687-92181 | Desulfococcus multivorans vrlR gene | CT009609 | 88 |
Numbers are nucleotide positions. Compl, complementary strand.
Data refer to identity and similarity at the amino acid level, except the value for vrlR, which refers to the similarity at the nucleotide sequence level.