TABLE 2.
Predicted products of ICEPdaSpa1-specific genes
| Genome region and predicted ORF or product (no. of amino acids) | Positions of ORF in ICEPdaSpa1a | Gene or protein homologue | GenBank accession no. for homologue gene | % Identity/% similarity to homologueb |
|---|---|---|---|---|
| rumB region | ||||
| TetA (400) | 11676-12878 (compl) | P. damselae subsp. piscicida tetracycline resistance protein, class D | BAA03719 | 66/78 |
| TetR (200) | 12959-13561 | E. coli Tet repressor, class D | 1A6I | 74/85 |
| TnpI (96) | 13951-14241 | Psychromonas ingrahamii IS3/IS911 transposase | ZP_01350213 | 62/80 |
| TnpII (243) | 14373-15104 | Psychromonas ingrahamii integrase | ZP_01350212 | 64/80 |
| Region between s035′ and ′s035 | ||||
| IS10 transposase (402) | 28188-29396 (compl) | Salmonella enterica serovar Typhi IS10 transposase | NP_058298 | 100/100 |
| Hot spot 2 | ||||
| Spa01 (909) | 52041-54770 | Psychromonas sp. strain CNPT3 putative helicase | ZP_01215992 | 40/60 |
| Spa02 (369) | 54767-55876 | No database matches with significant similarity | ||
| Spa03 (510) | 55889-57421 | Acidovorax sp. strain JS42 phospholipase D/transphosphatidylase | ZP_01383961 | 28/47 |
| Spa04 (377) | 57736-58869 | Desulfovibrio desulfuricans two-component transcriptional regulator, Fis family | ABB37621 | 38/61 |
| Hot spot 4 | ||||
| Spa05 (175) | 68950-69474 (compl) | No database matches with significant similarity | ||
| Spa06 (257) | 69487-70257 (compl) | Salmonella enterica hypothetical protein | AAG03008 | 38/52 |
| Hot spot 3 | ||||
| IS10 transposase (402) identical to the IS10 transposase listed above | 81904-83112 (compl) | Salmonella enterica serovar Typhi IS10 transposase | NP_058298 | 100/100 |
| Spa07 (831) | 83835-86330 | Deltaproteobacterium MLMS-1 heat shock protein Hsp70 | EAT04212 | 80/88 |
| Spa08 (489) | 86334-87803 | Deltaproteobacterium MLMS-1 AAA ATPase, central region | EAT04211 | 82/91 |
| Spa09 (553) | 87816-89477 | Deltaproteobacterium MLMS-1 hypothetical protein | EAT04215 | 68/82 |
| Spa10 (235) | 89470-90177 | No database matches with significant similarity | ||
| Spa11 (464) | 90286-91680 | Deltaproteobacterium MLMS-1 peptidoglycan-binding domain 1 | EAT04214 | 39/51 |
| ψvrlR | 91687-92181 | Desulfococcus multivorans vrlR gene | CT009609 | 88 |
a
Numbers are nucleotide positions. Compl, complementary strand.
b
Data refer to identity and similarity at the amino acid level, except the value for vrlR, which refers to the similarity at the nucleotide sequence level.