TABLE 2.
Identification by MS-MS of prominent proteins present in all cell extracts of L. casei 334 revealed by 2D PAGE (Fig. 4)
| Spot | Protein | Accession no. | Mol wt (103) | pI |
|---|---|---|---|---|
| 1 | Translation elongation factor (GTPase) | YP_807686 | 76.7 | 4.77 |
| 2 | Chaperone protein (HSP70) | YP_806781 | 67.4 | 4.77 |
| 3 | PTS enzyme I (EC 2.7.3.9) | YP_806973 | 63.3 | 5.00 |
| 4 | Pyruvate kinase (EC 2.7.1.40) | YP_806585 | 62.7 | 5.20 |
| 5 | Chaperonin GroEL (HSP 60) | YP_807425 | 57.4 | 4.89 |
| 6 | AICAR transformylase (EC 2.1.2.3) | YP_806961 | 54.7 | 5.40 |
| 7 | GTPase elongation factor(s) | YP_806555 | 43.4 | 4.87 |
| 8 | Oxaloacetate decarboxylase (EC 4.1.1.3) | YP_807056 | 51.8 | 5.10 |
| 9 | 3-Phosphoglycerate kinase (EC 2.7.2.3) | YP_806208 | 42.1 | 5.64 |
| 10 | Enolase (EC 4.2.1.11) | YP_806210 | 47.0 | 4.73 |
| 11 | DNA polymerase/clamp unit (EC 2.7.7.7) | YP_805310 | 41.3 | 4.64 |
| 12 | Elongation factor fragment | YP_805520 | 40.1 | 4.84 |
| 13 | Tagatose-1,6-bisphosphate aldolase (EC 4.1.2.40) | YP_807760 | 36.2 | 5.25 |
| 14 | Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) | YP_806207 | 36.6 | 5.68 |
| 15 | l-Lactate dehydrogenase (EC 1.1.1.27) | YP_807714 | 35.4 | 5.24 |
| Catabolite control protein (CcpA) | YP_806087 | 36.3 | 5.26 | |
| 16 | UDP-glucose-4-epimerase (EC 5.1.3.2) | YP_805947 | 36.2 | 5.69 |
| 17 | Triose phosphate isomerase (EC 5.3.1.1) | YP_806209 | 26.8 | 4.87 |
| 18 | dTDP-glucose pyrophosphorylase (EC 2.7.7.24) | YP_807212 | 32.2 | 5.48 |
| 19 | Phosphoglycerate mutase (EC 5.4.2.1) | YP_807326 | 25.8 | 5.43 |
| 20 | DNA-binding protein (Ssb) | YP_805318 | 21.0 | 5.14 |
| 21 | ATP-dependent Clp protease fragment/subunit | YP_806204 | 21.4 | 5.09 |
| 22 | PTS phosphocarrier protein (HPr) | YP_806974 | 9.1 | 4.91 |
| 23 | Chaperonin GroES (Cpn 10) | YP_807426 | 9.9 | 4.93 |