TABLE 1.
Effect of recA mutations on relative cell viability following induction of wild-type or mutant Y. pestis topoisomerase I
| Strain | Avg relative viability ± SDa
|
|||
|---|---|---|---|---|
| pAYTOP with:
|
pAYTOP-G122S with:
|
|||
| 0.00006% arabinose | 0.0002% arabinose | 0.00006% arabinose | 0.0002% arabinose | |
| BW27784 | 0.87 ± 0.30 | 0.45 ± 0.20 | 0.065 ± 0.007 | 0.020 ± 0.003 |
| YT101 | 1.05 ± 0.15 | 0.52 ± 0.19 | 3.9 × 10−4 ± 1.5 × 10−4 | 6.0 × 10−5 ± 3.3 × 10−5 |
| JHS1 | 0.71 ± 0.40 | 0.19 ± 0.07 | 9.2 × 10−3 ± 5.3 × 10−3 | 1.1 × 10−4 ± 3.4 × 10−5 |
a
The relative viability was measured as the ratio of viable counts determined 2 h after the addition of 0.00006 or 0.0002% arabinose versus the viable counts from control cultures not treated with arabinose. The results represent the average from three experiments.