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. 2008 Feb 29;190(9):3344–3352. doi: 10.1128/JB.01800-07

FIG. 4.

FIG. 4.

MS analysis of VGDANPALQK cross-link to show the RNA sequence where it binds through the cytosine residue. (A) Spectral range (m/z 1000 to 2600) which includes the peptide stretch alone (m/z 1012.48) and its different cross-linked form. (i) The VGDANPALQK peptide only and the same peptide cross-linked with one C residue are shown. (ii) MS spectrum of the signal at m/z 2276.20, derived from the VGDANPALQK peptide, where it remains attached with the UGUC nucleotide stretch of domain V. (iii) The same peptide, while attached to the UUGUC nucleotide stretch of domain V, gives a signal at m/z 2580.48. (B) The deduced oligonucleotide sequence of domain V RNA is shown; the last C residue of the 3′ UUGUC 5′ stretch interacts with the peptide.