FIG. 3.

Characterization of gonococcal transcriptional fusions. Expression of N. gonorrhoeae rmp, fur, and tonB GFP transcriptional fusions during in vitro growth. N. gonorrhoeae strains that expressed the transcriptional fusions F62-rmpGFP, F62-furGFP, and F62-tonBGFP were grown in iron-replete and iron-depleted conditions; samples were taken at every hour (so indicated), and fluorescence was measured by FACS analysis. The fluorescence mean was measured by using CellQuest software (version 3.3) and is expressed as the ratio of rmp, fur, and tonB genes (iron depleted and iron replete). The bars indicate the fold change ratios: white bars for the rmp gene (not visible since this was 1.0), hatched bars for the fur gene, and black bars for the tonB gene Data represent mean ratios ± the SD of three independently performed experiments.