TABLE 1.
Primers used in this study
| Primer pair sequence (5′-3′)a | Amplified gonococcal sequence |
|---|---|
| F, CGGGATCCACAGGCGAGGATAGGGTTTGT | 121-bp fur promoter fragment |
| R, CCAAGCTTGACAGGGTAAAATACCGCTTA | |
| F, CGGGATCCGAATTCCTATCCGATTTGCCG | 100-bp rmp promoter fragment |
| R, CCAAGCTTTTATTCCCTCATTAGATTTGTA | |
| F, CGGGATCCCACGGTTTGCCTATGAATGG | 91-bp tonB promoter fragment |
| R, CCGGATCCGGGCATAATAATAGCAACAATTCC | |
| F, GAAACCATTTCCCTGTCTGC | 347-bp rmp internal fragment |
| R, GTTACGATGCTGCGGATTTT | |
| F, GAAATGTTATCGAATTTGTCG | 498-bp tonB internal fragment |
| R, GTAAAGGATTGCCTTTGCT | |
| F, CGCGTTTGAAGATTTTGGAT | 348-bp fur internal fragment |
| R, TGCACACGCCGTACATATAAA | |
| F, GGATAAATTGCCCGAAGGTT | 347-bp tbpA internal fragment |
| R, CACGGAGGGTGAAGTGTTTT | |
| F, TATCCGATACGCACTGCTTG | 349-bp fbpA internal fragment |
| R, CAGTGCCACCCAGTCTTTTT | |
| F, GCGGTAGTCGATGTAATAGG | 407-bp sodB internal fragment |
| R, CAAACCTACATCACCAACCT |
a
F, forward primer; R, reverse primer.