TABLE 3.

Expression of hypothetical gonococcal genes by microarray analysis during infection of endocervical epithelial cells by N. gonorrhoeae strain F62

ORFa	IRb	Hypothetical gene product	Log2 ratioc at:
			1 h		2 h		4 h		8 h
			TOT	INT	TOT	INT	TOT	INT	TOT	INT
NG0021	+	Iron receptor; TonB dependent ferric siderophore receptor; similar to fhuE from E. coli	4.39	4.72	4.43	4.69	3.47	3.71	4.15	4.29
NG1536	+	Conserved hypothetical protein	4.31	4.31	4.60	4.20	3.53	3.04	4.01	3.70
NG2049	+	Neisseria-specific, integral membrane protein	3.81	3.97	4.06	3.68	3.40	2.77	3.46	3.25
NG1189	-	hsp33; heat shock protein Hsp33 chaperonin	2.67	3.26	2.77	3.52	2.05	2.64	2.80	2.84
NG1529	+	ftsA; cell division protein	2.42	2.35	2.31	2.59	1.75	1.65	2.20	2.04
NG1823	-	rplO; 50S ribosomal protein L15	1.83	1.64	1.37	1.27	1.59	1.02	1.09	0.84
NG1976	+/-	Neisseria-specific protein	1.78	1.91	1.65	2.11	1.15	1.30	1.66	1.74
NG0869	-	dedA; probable MSD protein in ABC transport	1.55	1.61	1.05	1.17	0.73	0.45	0.54	0.38
NG0044	-	accC; acetyl-CoA carboxylase	1.73	1.85	2.25	2.12	1.52	1.27	1.27	1.25
NG1376	+	conserved hypothetical protein, possible ABC solute binding protein	1.73	1.82	1.69	2.01	0.97	0.87	1.58	1.43
NG0959	+	Conserved hypothetical protein	1.39	1.00	1.64	0.96	1.58	0.43	0.85	0.53
NG0108	+	Conserved hypothetical protein (oxidoreductase)	1.19	1.73	1.33	1.56	0.47	0.67	0.16	0.43

^aThe numbers denote the ORF numbers assigned by the gonococcal genome sequence project (http://www.genome.ou.edu/gono.html).

^bIR, iron response: +, iron-repressed genes; -, iron-activated genes.

^cThe indicated values are the ratios expressed as log₂ of the intensity of the mean of the spots that represented the indicated gene divided by the mean of spots that represented the rmp gene of the total associated (adhered plus intracellular) (TOT) and intracellular only (INT) gonococci after infection of endocervical epithelial cells at 1, 2, 4, and 8 h.