TABLE 4.
Expression of representative gonococcal genes with a known function by microarray analysis during uncomplicated gonorrhea in women
| Gene (ORF)a | IRb | Gene product | Median log2 ratio (mean ± SEM)c |
|---|---|---|---|
| tonB (NG1379) | + | Transport and binding proteins | 3.59 (2.86 ± 0.43) |
| fbpB (NG0216) | + | Iron-uptake permease inner membrane protein, MSD protein in ABC transport system | 0.78 (0.48 ± 0.23) |
| exbD (NG1377) | + | Transport protein | 2.09 (2.03 ± 0.46) |
| tbpA (NG1495) | + | Transferrin binding protein A | 2.55 (2.02 ± 0.56) |
| fbpA (NG0217) | + | Periplasmic iron-binding protein, ABC solute binding protein | 0.25 (0.2 ± 0.17) |
| fumC (NG1029) | + | Fumarate hydratase | 0.83 (0.29 ± 0.28) |
| fetA (NG2093) | + | Iron-regulated outer membrane protein | 0.09 (0.13 ± 0.32) |
| fur (NG1779) | + | Ferric uptake regulatory protein | 0.2 (0.23 ± 0.35) |
a
The numbers in parentheses denote the ORF numbers assigned by the gonococcal genome sequence project (http://www.genome.ou.edu/gono.html).
b
IR, iron response: +, iron-repressed genes.
c
Data were obtained from clinical samples. The indicated values are the ratios expressed as log2 of the intensity of the mean of the spots that represented the indicated gene divided by the mean of spots that represented the rmp gene from the same clinical sample.