TABLE 5.
Expression of hypothetical gonococcal genes by microarray analysis during uncomplicated gonorrhea in women
| ORFa | IRb | Hypothetical gene product | Median log2 ratio (mean ± SEM)c |
|---|---|---|---|
| NG0021 | + | Iron receptor; TonB dependent ferric siderophore receptor; similar to fhuE from E. coli | 2.33 (2.21 ± 0.46) |
| NG1536 | + | Conserved hypothetical protein | 1.93 (1.40 ± 0.52) |
| NG2049 | + | Neisseria-specific, integral membrane protein | 0.9 (0.80 ± 0.31) |
| NG1189 | - | hsp33; heat shock protein Hsp33 chaperonin | 2.33 (2.19 ± 0.59) |
| NG1529 | + | ftsA; cell division protein | 0.33 (0.35 ± 0.18) |
| NG1823 | - | rplO; 50S ribosomal protein L15 | 0.05 (0.13 ± 0.17) |
| NG1976 | +/- | Neisseria-specific protein | 0.70 (0.45 ± 0.18) |
| NG0869 | - | dedA; probable MSD protein in ABC transport | 0.07 (0.17 ± 0.35) |
| NG0044 | - | accC; acetyl coenzyme A carboxylase | 0.45 (0.17 ± 0.13) |
| NG1376 | + | Conserved hypothetical protein, possible ABC solute binding protein | 0.05 (0.06 ± 0.12) |
| NG0959 | + | Conserved hypothetical protein | 0.53 (0.30 ± 0.3) |
| NG0108 | + | Conserved hypothetical protein (oxidoreductase) | 0.09 (0.12 ± 0.17) |
a
The numbers denote the ORF numbers assigned by the gonococcal genome sequence project (http://www.genome.ou.edu/gono.html).
b
IR, genes regulated by iron: +, iron-repressed genes; -, iron-activated genes.
c
Data were obtained from clinical samples. The indicated values are the ratios expressed as log2 of the intensity of the mean of the spots that represented the indicated gene divided by the mean of spots that represented the rmp gene from the same clinical sample.