FIG. 1.
Effect of DivIVA depletion on cell shape and polar growth of Corynebacterium glutamicum. (A) Quantitative Western blot of DivIVA from different exponentially grown C. glutamicum strains. Lane 1, strain R31; lane 2, strain R31 plus 4%Suc; lane 3, strain GNTD (PgntP-divIVACg); lanes 4 and 8, strain GNTD grown in 4%Suc; lane 5, strain LACD (Plac-divIVACg); lanes 6 and 9, strain LACID (Plac-divIVACg lacIq); lane 7, strain AG1 (multicopy divIVACg). Ten micrograms of cell extract proteins was used in lanes 8 and 9; otherwise, 1 μg was loaded per lane. (B) Phase-contrast and Van-FL staining of exponentially growing cells. The typical morphology of C. glutamicum R31 was converted to a coccoid morphology (no polar growth) in strain LACID, which is depleted of DivIVA. In C. glutamicum LACID the zonal PG synthesis is absent whereas strain AG1 (multicopy divIVACg) grows apically and forms many apparently incomplete septa. Wt, wild type.