FIG. 4.

Interactions between different DivIVA proteins from gram-positive bacteria analyzed in a bacterial two-hybrid system. All combinations between bait (abscises) and prey (legends) are shown. The basal growth detected in negative controls (E. coli BacterioMatch II transformed with the empty plasmids) was considered as the background used to represent the data. The values are the means of four independent experiments; standard deviations are indicated on the bar top. Note that the DivIVA proteins from actinomycetes interact with each other, but they do not interact with DivIVAs from B. subtilis or S. pneumoniae.