FIG. 5.
DivIVA arrives at mid-cell at a late stage of cell division in C. glutamicum. (A) Immunofluorescence (IFL) microscopy using anti-DivIVA antiserum and DAPI staining of nucleoids in C. glutamicum R31. Arrows show that DivIVA localizes at the septum in cells with visibly segregated nucleoids. Size bar, 1 μm. (B) Fluorescence microscopy (EGFP2) and Van-650/665 staining of C. glutamicum R33, carrying an extra chromosomal copy of divIVACg-egfp2 under the control of Pdiv (Pdiv-divIVACg-egfp2). Arrows indicate the cells in which the biosynthesis of new PG (visualized by Van-650/660 staining) has preceded septal localization of DivIVA-EGFP2 (EGFP2 fluorescence). Size bar, 1 μm.