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. 2008 Feb 29;190(9):3098–3109. doi: 10.1128/JB.01709-07

FIG. 6.

FIG. 6.

(A) Collagen binding activity of the wild-type (WT) strain VT1169 or the emaA mutant strain complemented with pKMΔ70-144, pKMΔ145-185, or pKMΔ186-206 (plasmids containing an in-frame deletion corresponding to amino acids 70 to 144, 145 to 185, or 186 to 206) measured by ELISA. The data are represented as a percentage binding relative to the emaA mutant strain complemented with the full-length sequence (pKM9). (B) Immunodot blot of the whole-cell lysates from 2 × 108 CFU of the wild-type (WT) strain VT1169 or the emaA mutant strain complemented with the empty plasmid (pKM1), the full-length sequence (pKM9), or pKMΔ145-185 (a plasmid containing a deletion corresponding to amino acids 145 to 185).