TABLE 2.
Oligonucleotides used for the generation of SmaI sites for deletion analysis of EmaA
| Oligonucleotide | Sequence (5′-3′)a | Positionsb |
|---|---|---|
| SmaI208f | AACAATTCTGTGAAACCCGGGGGGGCAGAAGCCTCC | 832-867 |
| SmaI208r | GGAGGCTTCTGCCCCCCCGGGTTTCACAGAATTGTT | 832-867 |
| SmaI433f | TTATTCTAAAGCTACCCCCGGGCTTGCCATGGCCTTAGG | 1056-1094 |
| SmaI433r | CCTAAGGCCATGGCAAGCCCGGGGGTAGCTTTAGAATAA | 1056-1094 |
| SmaI556f | GTCAATCTGCAGCACCCGGGGATTATTCTACTG | 1181-1213 |
| SmaI556r | CAGTAGAATAATCCCCGGGTGCTGCAGATTGAC | 1181-1231 |
| SmaI619f | TCAATCAAGCTTCGCACCCGGGGCTTCTGCTAC | 1242-1274 |
| SmaI619r | GTAGCAGAAGCCCCGGGTGCGAAGCTTGATTGA | 1242-1274 |
| SmaI1159f | CATTGTCAACAGTGTCGATCCCGGGCAATCTAAATTTACTG | 1779-1819 |
| SmaI1159r | CAGTAAATTTAGATTGCCCGGGATCGACACTGTTGACAATG | 1779-1819 |
a
Generated SmaI sites are indicated in boldface.
b
That is, the positions of the oligonucleotides in the emaA sequence (accession number AY344064).