TABLE 3.
Oligonucleotides used to generate substitution mutations in the DNA sequence coding for amino acids 162-GIAIG-166
| Oligonucleotide | Sequence (5′-3′)a | Positionsb |
|---|---|---|
| I163Ef | GAGCAGAAAAAAATGGCGGTGAGGCAATCGGTATAGCTTCCAG | 1106-1148 |
| I163Er | CTGGAAGCTATACCGATTGCCTCACCGCCATTTTTTTCTGCTC | 1106-1148 |
| I165Ef | GAAAAAAATGGCGGTATTGCAGAGGGTATAGCTTCCAGATCATCAG | 1111-1156 |
| I165Er | CTGATGATCTGGAAGCTATACCCTCTGCAATACCGCCATTTTTTTC | 1111-1156 |
| A164Df | GAGCAGAAAAAAATGGCGGTATTGATATCGGTATAGCTTCCAG | 1106-1148 |
| A164Dr | CTGGAAGCTATACCGATATCAATACCGCCATTTTTTTCTGCTC | 1106-1148 |
| I163E-A164Df | GCAGAAAAAAATGGCGGTGAGGACATCGGTATAGCTTCCA | 1108-1147 |
| I163E-A164Dr | TGGAAGCTATACCGATGTCCTCACCGCCATTTTTTTCTGC | 1108-1147 |
| I163E-A164D-I165Ef | CAGAAAAAAATGGCGGTGAGGACGAGGGTATAGCTTCCAGATC | 1109-1153 |
| I163E-A164D-I165Er | GATCTGGAAGCTATACCCTCGTCCTCACCGCCATTTTTTTCTG | 1109-1153 |
| I163A-I165Af | AGAAAAAAATGGCGGTGCTGCAGCCGGTATAGCTTCCA | 1110-1147 |
| I163A-I165Ar | TGGAAGCTATACCGGCTGCAGCACCGCCATTTTTTTCT | 1110-1147 |
| G162Af | GCAGAAAAAAATGGCGCTATTGCAATCGGTATAGCTTC | 1108-1145 |
| G162Ar | GAAGCTATACCGATTGCAATAGCGCCATTTTTTTCTGC | 1108-1145 |
| G162Sf | TTTCCAGAGCAGAAAAAAATGGCAGTATTGCAATCGGTATAG | 1100-1141 |
| G162Sr | CTATACCGATTGCAATACTGCCATTTTTTTCTGCTCTGGAAA | 1100-1141 |
a
Substituted codons are indicated in boldface.
b
That is, the positions of the oligonucleotides in emaA sequence (accession number AY344064).