TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| E. coli strains | ||
| CC118 | recA1 supE44 endA1 hsdR17 (rK− mK−) gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+lacIqlacZΔM15] | 80 |
| CC118λPm::lacZ | CC118, lysogenized with a λPm::lacZ fusion | 33 |
| BL21(DE3) | Carries T7 RNA polymerase under the control of lacUV5 promoter | Novagen |
| DH5α | λ− φ80dlacZΔM15 Δ(lacZYA-argF)U196 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | 25 |
| Plasmids | ||
| pJLR100 | Apr, Pm cloned in pEMBL9 | 58 |
| pCMX2 | Tcr, pSELECT derivative containing the wild-type xylS gene | 38 |
| pERD103 | Kmr, derivative containing the wild-type xylS gene | 56 |
| pLOW2 | Kmr, pACYC177 derivative, low-copy-no. cloning vector | 26 |
| pLOW2::XylS | pLOW2 derivative containing the xylS gene cloned as an EcoRI-XbaI fragment | 62 |
| pJLR107 | Apr, Pm::lacZ in pMD1405 | 58 |
| pMD::Pm245 | Apr, Pm245::lacZ in pMD1405 | 16 |
| pET16b::XylS-C | Apr, pET16b derivative used to produce His-tagged XylS C-terminal domain | Domínguez-Cuevas et al., unpublished |
| pET16b::rpoS | Apr, pET16b derivative used to produce His-tagged P. putida σ38 | This work |
| pET16b::rpoH | Apr, pET16b derivative used to produce His-tagged P. putida σ32 | This work |
| pET16b::XylS-N | Apr, pET16b derivative used to produce His-tagged XylS N-terminal domain | This work |
| pGP1-2 | Kmr, contains an inducible T7 RNA polymerase gene | 70 |
| pBBR1::XylS-C | Gmr, pBBR1MSC5 derivative containing XylS-C | This work |
a
Apr, Kmr, and Tcr indicate resistance to ampicillin, kanamycin, and tetracycline, respectively.