FIG. 3.

Transcriptional analyses of dcw cluster genes in Prosthecobacter. Five-microliter aliquots of PCR products were loaded on a 2% agarose gel. The template for PCR was the cDNA sample (+) or a control sample processed without reverse transcriptase (−). A 100-bp ladder (Invitrogen) was loaded on the left side; the numbers refer to fragment lengths in base pairs. The expected sizes of the fragments were 296 bp (ftsZ), 435 bp (ftsA), 354 bp (ftsQ), and 283 bp (ddl). Transcription was detected for all investigated genes in P. dejongeii (shown) and P. vanneervenii.