FIG. 2.

C. burnetii metabolic fitness in supplemented P-25 buffer and CCM. The ability of P-25 buffer supplemented with 5 mM glutamate (broken line) and CCM (solid line) to sustain C. burnetii metabolic fitness was directly compared by examining de novo protein synthesis in labeling buffer following preincubation in these solutions for 2, 6, and 24 h. Following preincubations, C. burnetii was labeled with [³⁵S]Cys-Met in labeling buffer (pH 4.5) for 3 h and then subjected to scintillation counting (A) and SDS-PAGE and autoradiography (B). In panel A, results are expressed as the relative increase in radiolabel incorporation compared to bacteria that were directly labeled in labeling buffer (pH 7.0) (negative control). The 0-h time point represents the relative increase in radiolabel incorporation by C. burnetii cells that were directly labeled in labeling buffer (pH 4.5) without preincubation. Asterisks indicate statistically significant differences (P < 0.05) between CCM and supplemented P-25 buffer. A representative autoradiogram is shown. MW, molecular weight (in thousands).