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The BTB/POZ domain of LAZ3/BCL6 directly interacts with SMRT in GST pulldown assays. In vitro translated and 35S-methionine-labeled SMRT (lane 1, I represents 40% of the SMRT amount used in the pulldown experiments) was incubated with either GST alone (lane 2), or GST-LAZ(BTB/POZ) (lane 4). Interaction between SMRT and GST-RARα is shown as a positive control (lane 3). Molecular masses are indicated on the left.
