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. 1997 Sep 30;94(20):10774–10779. doi: 10.1073/pnas.94.20.10774

Figure 2.

Figure 2

M1 and M10 mutants have mutations in codon 149 of the RAM1 gene. (A) Nucleotides 424–468 of the RAM1 gene from wild-type (JRY5312) and M1 and M10 mutant strains (JRY5388 and JRY5389). The sequences of the ram1–102 and ram1–103 alleles, in M1 and M10, respectively, were identical and differed from the wild-type RAM1 sequence in having an A at nucleotide 446 rather than a G, which changed codon 149 to GAA (a glutamate codon, underlined) from GGA (a glycine codon). (B) Predicted sequence of amino acids 135–172 of mutant Ram1p (M1 and M10 yeast FTβ), wild-type Ram1p (yeast FTβ; ref. 63 and this study), and homologous regions from human farnesyltransferase β-subunit (human FTβ; ref. 32) and tomato farnesyltransferase β-subunits (tomato FTβ; ref. 54). The G149E amino acid substitution in the ram1–102 and ram1–103 mutants (underlined) was in a highly conserved position in farnesyltransferase.