Table 1.

ACS matches and mutations

ACS match	Location, bp	Mutant version	Positions altered
ATCTATATTTT	31–41	ATCTATATaaT	39, 40 T → A
TTATATGTTTA	169–159	TTATATGaaTA	161, 162 T → A
TTTTATATGTT	171–161	TTTTATATGaa	161, 162 T → A
TTTTTTATTTT	241–231	TTTTTTAaaTT	233, 234 A → T
TTTGAAGTTTT	75–85	TTTGAAGTaaT	83, 84 T → A
TTAAATGTTTT	104–94	TTAAATGTggT	95, 96 A → C
GTTTATATCTT	62–72	GTTTATAgCgT	69, 71 T → G

Matches to the ACS in the 350-bp HpaI–EcoRI fragment. Positions that differ from the ACS are underlined. Alterations in the mutant versions are indicated as lowercase type. All matches are shown as the T-rich strand.