Table 1.
RT activity in conditioned medium from packaging cell lines
| Cell | Total RT, dpm | RT in pellet, % of total RT | RT in supernatant after centrifugation, % of total RT |
|---|---|---|---|
| 293GP | 3.8 × 107 | 50 | 1.8 |
| 293GP (mock transfection) | 3.9 × 107 | 25 | 2.2 |
| 293GP LZRNL (mock transfection) | 4.4 × 107 | 36 | 0.3 |
| 293GP LZRNL (amphotropic env) | 4.7 × 107 | 53 | 2.3 |
| 293GP LZRNL (VSV-G env) | 5.8 × 107 | 33 | 2.9 |
Total RT in sample before pelleting is expressed in dpm units. RT in pellet is the percent of total RT recovered in the pellet after centrifugation.