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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2007 Dec 14;228(1):93–104. doi: 10.1016/j.taap.2007.11.032

FIG 6.

FIG 6

Suppression of vincristine-induced JNK and Bcl-2 phosphorylation as well as PARP cleavage by pre-treatment with non-lethal heat stress. (A) Immunoblot of heat stress protein hsp 70 (HSP 70) in protein lysates from cultures exposed to the indicated concentrations of vincristine for 24 h without prior heat stress (−HS), or following limited, non-lethal, heat stress (+NL HS; 30 min @ 43°C), or following extended, lethal heat stress, (+L HS; 1 h @ 43°C). (B) Protein lysates from cultures treated with the indicated concentrations of vincristine without prior heat stress (−HS) or subjected to limited heat stress (+NL HS) were blotted for phosphorylated Bcl-2 thr 56 (p-Bcl-2, upper band, double asterik), phosphorylated JNK (p-JNK) or total JNK protein (JNK; loading control) at 2 h following drug addition and PARP at 24 h following drug addition. Percent of PARP that is cleaved (arrow) as determined by densitometry is indicated below each lane.