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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2007 Nov 9;85(3-4):69–80. doi: 10.1016/j.prostaglandins.2007.10.005

Figure 7.

Figure 7

Two dimensional electrophoresis of unpurified SpV-1/SpV-2 rabbit reticulocyte lysate co-translation products. Panel A, upper horizontal lane is a representative lane from the same gel as used for the 2D analysis showing a Coomassie blue protein stain of the first dimension (Native-PAGE). The lower panel is the naphthol blue black protein stain of the completed 2D separation transfer. Panel B is the Pafah1b2 immunostain using chemiluminescent substrate of the 2D western blot showing the Alpha-2, SpV-2 and SpV-1 monomers.