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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2007 Nov 9;85(3-4):69–80. doi: 10.1016/j.prostaglandins.2007.10.005

Table 2.

Primer sets used for cDNA amplification (Part A), RACE experiments (Part B), and In Vitro translation (Part C).

A.
Set Target Use Forward Reverse RNA source
1 Ex-1 to AE-C Amplification 41-f 31992-r Tissues, 1st Strand cDNA
2 Ex-1 to AE-B Amplification 41-f 27384-r Tissues, 1st Strand cDNA
3 Ex-1 to AE-D Amplification 41-f 33747-r Tissues, 1st Strand cDNA
4 Ex-1 to AE-A Amplification 41-f 26537-r Tissues, 1st Strand cDNA
5 Ex-1 to Ex-6 Amplification 41-f 23671-r Tissues, 1st Strand cDNA
B.
RACE RACE Primary RACE Primers Nesting RACE Primers RNA
Set Target Type Forward Reverse Forward Reverse source
6 AE-C/AE-D 3′ RACE 19515-f UPM 31755-f NUP-A Kidney/Testis
7 AE-C 5′ RACE UPM 31992-r NUP-A 31723-r Testis
8 AE-B 3′ RACE 19515-f UPM 27330-f NUP-A Testis
9 AE-B 5′ RACE UPM 31723-r NUP-A 27384-r Testis
10 AE-D 3′ RACE 19515-f UPM 27330-f NUP-A Testis
11 I8 (SpV-5) 3′ RACE 19515-f UPM 30770-f NUP-A Testis
12 AE-A 3′ RACE 19515-f UPM 25188-f NUP-A Testis
C.
Set Target Use Forward Reverse Template
13 Ex-2 to Ex-6 In Vitro Translation T7-to-Met1-f 23671-r Purified Human Testis Alpha-2 cDNA
14 Ex-2 to AE-C In Vitro Translation T7-to-Met1-f 31723-r Purified Human Testis SpV-1 or SpV-2 cDNA

Key: UPM = Universal primer mix; a mix of Long UP (0.4uM) and short UP (2uM) primers. NUP-A = Nest-UP-A