Figure 1.

Comparison of anti-Id titers following immunization using CpG ODN or DNA as an adjuvant. 38C13 Id IgM was conjugated to KLH using gluteraldehyde to produce Id-KLH. A total of 50 μg Id-KLH was mixed with adjuvant in aqueous solution and injected subcutaneously into naive 6- to 8-week-old female C3H mice in a volume of 0.2 ml. Each mouse received a single immunization. Blood was obtained weekly, and serum was evaluated for the presence of anti-Id IgG by ELISA. Normal mouse serum supplemented with a known concentration of monoclonal anti-Id was used as a standard. Three mice were included in each group.