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Comparison of ipsilateral and contralateral injection. Mice were immunized and anti-Id levels determined as in Fig. 1. All mice received 50 μg Id-KLH and 50 μg CpG ODN 1758. Mice were injected with Id-KLH and CpG ODN 1758 mixed together (ipsilateral) or were injected on the right flank with Id-KLH and CpG ODN 1758 on the left flank (contralateral) to assess whether the adjuvant effect of CpG ODN is systemic or local.
