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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: J Neurosci Methods. 2007 Apr 18;164(1):116–119. doi: 10.1016/j.jneumeth.2007.04.007

Table 2.

Zebra Finch Sex by PCR Mastermix Preparation

Component Stock Concentration Volume to Add Final Concentration
PCR Buffer1 10 X 100 mcL 1 X
MgCl2 25 mM 100 mcL 2.5 mM
dNTP mix 10 mM each 20 mcL 200 mcM each
W1 Primer 100 mcM 100 mcL 1 mcM
W2 Primer 100 mcM 100 mcL 1 mcM
Z1 Primer 100 mcM 100 mcL 1 mcM
Z2 Primer 100 mcM 100 mcL 1 mcM
Sterile H2O --- 374 mcL QS to 1000 mcL
Taq DNA Polymerase2 5 U/mcL 6 mcL 0.75 U/22.5 mcL
1

500 mM KCl, 100 mM Tris-Cl (pH = 8.3)

2

Vortex mix other components before adding Taq. Mix Taq by inversion.

3

After preparation dispense into aliquots and store at −20 C.