Table 5.
Cross reactive intralesional T-cell clones from RHD patients
| Patients # | Cross reactive T-cell clones | CD4 | CD8 | Antigen recognition M5 protein peptides / Heart tissue derived proteins or Cardiac myosin (LMM) peptides |
|---|---|---|---|---|
| 1 | 1.1.27 | + | M5(11–25) | |
| Myocardium protein: >150 kDa to 44 kDa | ||||
| 3 | 3.1.79 | + | M5(111–130) | |
| Mitral valve proteins: 54–53 kDa/pI 7.76; 52 kDa/pI 4.3 | ||||
| LMM 31(1686–1703); LMM 49 (1921–1937) | ||||
| 4 | 4.1.18 | + | M5(81–103) and M5(163–177) | |
| Aortic valve proteins: 100–115–120 kDa; 43–46–50 kDa | ||||
| 5* | 5.1.31 | + | M5(62–82) | |
| Myocardium protein: 90–150 kDa | ||||
| Aortic valve protein: 43–65 kDa | ||||
| 7 | 7.1.1 | + | M5(81–96); M5(83–103) | |
| Myocardium protein: ≥150 kDa | ||||
| Aortic valve proteins : 90–150 kDa; 43–65 kDa | ||||
| 8 | 8.1.2 | + | M5(81–96) | |
| Myocardium protein: 95–150 kDa | ||||
| 10 | 10.2.13 | + | Mitral valve proteins: 58; 37 kDa/pI 5.12 | |
| LMM14(1400–1417); LMM36(1751–1768); | ||||
| LMM38(1777–1795); LMM40(1803–1834) |
Note: T-cell clones were derived from mitral or aortic valve, except T-cell clone 5.1.31 (*) that was derived from left atrium (LA). Sequences of recognized peptides: M5 protein (Manjula et al, 1985; Phillips et al, 1981; Guilherme et al, 1995, 2000 and 2001): 11–25-QRAKEALDKYELENH; 62–82-LERKTAELTSEKKEHEAENDK; 81–96-DKLKQQRDTLSTQKET; 83–103-LKQQRDTLSTQKETLEREVQN; 111–130-TRQELANKQQESKENEKALN; 163–177- ETIGTLKKILDETVK. Cardiac myosin (LMM-light meromyosin) peptides (Diederich et al, 1989; Galvin et al, 2000; Fae et al, 2006): LMM14-(1400–1417) SQSELESSQKEARSLSTE; LMM31-(1686–1703) ELEELRAVVEQTERSRKL; LMM36-(1751–1768) RNAEEKAKKAITDAAMMA; LMM-38 (1777–1795) TSAHLERMKKNMEQTIKD; LMM40-(1803–1840) EQIALKGGKKQLQKLEAR; LMM 49-(1921–1937) LEARVRELENELEAEQKR.