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. 2007 Dec;3(6):1007–1017.

Table 5.

Cross reactive intralesional T-cell clones from RHD patients

Patients # Cross reactive T-cell clones CD4 CD8 Antigen recognition
M5 protein peptides / Heart tissue derived proteins or Cardiac myosin (LMM) peptides
1 1.1.27 + M5(11–25)
Myocardium protein: >150 kDa to 44 kDa
3 3.1.79 + M5(111–130)
Mitral valve proteins: 54–53 kDa/pI 7.76; 52 kDa/pI 4.3
LMM 31(1686–1703); LMM 49 (1921–1937)
4 4.1.18 + M5(81–103) and M5(163–177)
Aortic valve proteins: 100–115–120 kDa; 43–46–50 kDa
5* 5.1.31 + M5(62–82)
Myocardium protein: 90–150 kDa
Aortic valve protein: 43–65 kDa
7 7.1.1 + M5(81–96); M5(83–103)
Myocardium protein: ≥150 kDa
Aortic valve proteins : 90–150 kDa; 43–65 kDa
8 8.1.2 + M5(81–96)
Myocardium protein: 95–150 kDa
10 10.2.13 + Mitral valve proteins: 58; 37 kDa/pI 5.12
LMM14(1400–1417); LMM36(1751–1768);
LMM38(1777–1795); LMM40(1803–1834)

Note: T-cell clones were derived from mitral or aortic valve, except T-cell clone 5.1.31 (*) that was derived from left atrium (LA). Sequences of recognized peptides: M5 protein (Manjula et al, 1985; Phillips et al, 1981; Guilherme et al, 1995, 2000 and 2001): 11–25-QRAKEALDKYELENH; 62–82-LERKTAELTSEKKEHEAENDK; 81–96-DKLKQQRDTLSTQKET; 83–103-LKQQRDTLSTQKETLEREVQN; 111–130-TRQELANKQQESKENEKALN; 163–177- ETIGTLKKILDETVK. Cardiac myosin (LMM-light meromyosin) peptides (Diederich et al, 1989; Galvin et al, 2000; Fae et al, 2006): LMM14-(1400–1417) SQSELESSQKEARSLSTE; LMM31-(1686–1703) ELEELRAVVEQTERSRKL; LMM36-(1751–1768) RNAEEKAKKAITDAAMMA; LMM-38 (1777–1795) TSAHLERMKKNMEQTIKD; LMM40-(1803–1840) EQIALKGGKKQLQKLEAR; LMM 49-(1921–1937) LEARVRELENELEAEQKR.