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. 1977 Feb;129(2):803–808. doi: 10.1128/jb.129.2.803-808.1977

Phototaxis mutants of Chlamydomonas reinhardtii.

R Hirschberg, R Stavis
PMCID: PMC235014  PMID: 838688

Abstract

A group of 31 mutants of Chlamydomonas reinhardtii with altered phototactic responses was isolated. Growth of all the mutants was normal and, with one exception, they had no gross morphological aberrations. One mutant produced supernumerary flagella. The mutants can be subdivided into five phenotypic classes on the basis of their abnormal responses: slow positive, no reponse, slow negative, fast negative, and variable. Random motility of the mutants was also characterized. About half were indistinguishable from wild type; the others exhibited "jerky" or "twirly" movements instead of relatively straight paths. These mutants can now be used for genetic and biochemical studies of the cellular basis of phototaxis.

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Selected References

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  1. Cavalier-Smith T. Basal body and flagellar development during the vegetative cell cycle and the sexual cycle of Chlamydomonas reinhardii. J Cell Sci. 1974 Dec;16(3):529–556. doi: 10.1242/jcs.16.3.529. [DOI] [PubMed] [Google Scholar]
  2. Gould R. R. The basal bodies of Chlamydomonas reinhardtii. Formation from probasal bodies, isolation, and partial characterization. J Cell Biol. 1975 Apr;65(1):65–74. doi: 10.1083/jcb.65.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hopkins J. M. Subsidiary components of the flagella of Chlamydomonas reinhardii. J Cell Sci. 1970 Nov;7(3):823–839. doi: 10.1242/jcs.7.3.823. [DOI] [PubMed] [Google Scholar]
  4. Hudock G. A., Hudock M. O. Phototaxis: isolation of mutant strains of Chlamydomonas reinhardi with reversed sign of response. J Protozool. 1973 Feb;20(1):139–140. doi: 10.1111/j.1550-7408.1973.tb06018.x. [DOI] [PubMed] [Google Scholar]
  5. Hyams J. S., Borisy G. G. Flagellar coordination in Chlamydomonas reinhardtii: isolation and reactivation of the flagellar apparatus. Science. 1975 Sep 12;189(4206):891–893. doi: 10.1126/science.1098148. [DOI] [PubMed] [Google Scholar]
  6. LEWIN R. A. Mutants of Chlamydomonas moewusii with impaired motility. J Gen Microbiol. 1954 Dec;11(3):358–363. doi: 10.1099/00221287-11-3-358. [DOI] [PubMed] [Google Scholar]
  7. McVittie A. Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol. 1972 Aug;71(3):525–540. doi: 10.1099/00221287-71-3-525. [DOI] [PubMed] [Google Scholar]
  8. Nultsch W., Throm G. Effect of external factors on phototaxis of Chlamydomonas reinhardtii. I. Light. Arch Microbiol. 1975 Apr 7;103(2):175–179. doi: 10.1007/BF00436346. [DOI] [PubMed] [Google Scholar]
  9. RANDALL J., WARR J. R., HOPKINS J. M., MCVITTIE A. A SINGLE-GENE MUTATION OF CHLAMYDOMONAS REINHARDII AFFECTING MOTILITY: A GENETIC AND ELECTRON MICROSCOPE STUDY. Nature. 1964 Aug 29;203:912–914. doi: 10.1038/203912a0. [DOI] [PubMed] [Google Scholar]
  10. Ringo D. L. Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas. J Cell Biol. 1967 Jun;33(3):543–571. doi: 10.1083/jcb.33.3.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ringo D. L. The arrangement of subunits in flagellar fibers. J Ultrastruct Res. 1967 Feb;17(3):266–277. doi: 10.1016/s0022-5320(67)80048-0. [DOI] [PubMed] [Google Scholar]
  12. Rubin R. W., Filner P. Adenosine 3',5'-cyclic monophosphate in Chlamydomonas reinhardtii. Influence on flagellar function and regeneration. J Cell Biol. 1973 Mar;56(3):628–635. doi: 10.1083/jcb.56.3.628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stavis R. L., Hirschberg R. Phototaxis in Chlamydomonas reinhardtii. J Cell Biol. 1973 Nov;59(2 Pt 1):367–377. doi: 10.1083/jcb.59.2.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stavis R. L. The effect of azide on phototaxis in Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1974 May;71(5):1824–1827. doi: 10.1073/pnas.71.5.1824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Witman G. B., Carlson K., Berliner J., Rosenbaum J. L. Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes. J Cell Biol. 1972 Sep;54(3):507–539. doi: 10.1083/jcb.54.3.507. [DOI] [PMC free article] [PubMed] [Google Scholar]

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