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. Author manuscript; available in PMC: 2009 Mar 15.
Published in final edited form as: Virology. 2007 Dec 3;372(2):291–299. doi: 10.1016/j.virol.2007.11.002

Figure 4.

Figure 4

The SIVsmD116N mutant does not integrate into cell chromosome. CEM×174 cells and macaque macrophages were infected with SIVsmE543 and SIVsmD116N viruses. Total cellular DNA harvested was subjected to Alu-PCR amplification. A. No integrated viral DNA was amplified in up to 25×104 CEM×174 T cells infected with integrase mutant SIVsmD116N, while integrated DNA was amplified well in just 1×103 CEM×174 T cells infected with SIVsmE543. B. In D116N infected macrophages, Alu-PCR demonstrated that no integrated viral DNA persisted for 20 days until the termination of the cell culture. While in WT infected macrophages, integrated DNA amplified well from day 1 to day 20. For the “Alu-” controls without first round PCR, no product was amplified.