Table 1.

Sequences of Used Primers

Primer	Sequence	Position*
Subclone-1	5′-GGTATTACCTCAAGGGTGGAAGGG-3′	3295–3318
Subclone-2	5′-TCGGCAGGTGATTTACTGCTG-3′	5918–5938
Mutagenic-1	5′-CCATCACACATCTGCATACTAATAATGGTGCCAATTTCACAT-3′
Mutagenic-2	5′-ATGTGAAATTGGCACCATTATTAGTATGCAGATGT GTGATGG-3′
SIV/DNA-5′	5′-CCGAACAGGGACTTGAAGG AGGTGA-3′	708–731
SIV/DNA-3′	5′-TTTGTTCCTGCCGCCCTTACTG-3′	894–915
SIV/Circle-5′	5′-GCAAGTGTGTGTTCCCATCTCTCC-3′	9310–9331
SIV/Circle-3′	5′-AGCTCTCCGTCGTGGTTTGTTC-3′	909–930
Alu1-1	5′-TAGTCGGGAGGCTGAGGCAGGAGAA-3′	172–196 (human Alu)
Alu1-2	5′-GTCATCCCACTGATGAGTCTGTGC-3′	207– 230 (LTR U3)
Alu2-1	5′-TGGAAGGGATTTATTACAATGAGAAAAGAC-3′	1–30
Alu2-2	5′-CTCGTCTTCCTGGGCTTCATCT-3′	164–185
SIV/5′	5′-CCGAACAGGGACTTGAAGGAGGTGA-3′	842–866
SIV/nef-tat-rev	5′-CTCTGGAGCACTGGTTGGAGGATCTG-3′	9011–9036
SIV/3′ env	5′-GCAAGAGCGCGATAAGCA GCTGATTC-3′	6616–6641
SIV/3′ vif	5′-CTGCTGCAAGTCCACCATGCCC ATC-3′	5485–5509
SIV/3′ gagpol	5′-CTGAACCTGTCGGAACTAATGGAGC-3′	1257–1281

^*They are referred to positions in SIVsmE543 sequence except Alu1-1 and mutagenic primers.