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. Author manuscript; available in PMC: 2009 Apr 4.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jan 31;368(2):408–413. doi: 10.1016/j.bbrc.2008.01.100

Figure 1. Developmental changes in Gβ and Gγ expression in the ventricle.

Figure 1

100 μg of total cell protein extracted from day 5 neonatal cardiomyocyte cultures, or freshly isolated adult rat cardiomyocytes (Panel A) or from ventricles from rats at the indicated ages (Panel B) or were subjected to SDS-PAGE and immunoblot analysis with the indicated antibodies. An extract from brain (100 μg) was included as a positive control in Panel A. Epitope specific immunoreactivity was established by immunoblotting with antibody complexed with competing antigen peptide; note smaller proteins detected by the Gγ3 antibody are non-specific. Arrow denote epitope specific bands, with positions of the molecular weight standards (in kDa) indicated.