Figure 6. Platelet PLA₂ quantity and activity.

(A) Western blot of platelet lysate from the patient demonstrates cPLA_2α protein of expected molecular weight but in diminished quantity compared with controls. Band intensity was quantified using Quantity One image analysis software (n = 3). Error bars represent ± SD. (B) PLA₂ activity in platelet lysate measured by hydrolysis of a radiolabeled substrate, l-3-phosphatidylcholine, 1-stearoyl-2-[1-¹⁴C] arachidonyl (¹⁴C-SAPC). Points represent mean values for each time point (n = 4; 2 separate experiments performed in duplicate: 2 control volunteers and 2 different collection days for patient). Error bars represent ± SD.