Figure 9. Plasma insulin levels and glucose-stimulated insulin secretion in IR^Δper and IR^Δwb mice.

(A) Glucose-stimulated insulin secretion of 13-week-old Control^Δper (filled squares; n = 8), IR^Δper (open squares; n = 5), Control^Δwb (filled triangles; n = 5), and IR^Δwb mice (open triangles; n = 8) on day 18. Values are mean ± SEM. *P ≤ 0.05 versus 0 min. (B) Percentage of β cell mass in 14-week-old Control^Δper (black bars; n = 4), IR^Δper mice (white bars; n = 4), Control^Δwb (dark gray bars; n = 3), and IR^Δwb mice (light gray bars; n = 3). Data was collected 30 days after starting the experiment. Values are mean ± SEM. *P ≤ 0.05, **P ≤ 0.01 versus control. (C) Immunohistochemical stainings of pancreatic islets in 14-week-old Control^Δper, IR^Δper, Control^Δwb, and IR^Δwb mice. Pancreatic tissues were stained for H&E, insulin, and glucagon. Magnification, ×100.