Abstract
Populations of symbiotic Rhizobium meliloti extracted from alfalfa nodules were shown by flow microfluorometry to contain a significant number of bacteroids with higher nucleic acid content than the free-living rhizobia. Bacteroids with lower nucleic acid content than the free-living bacteria were not detected in significant quantities in these populations. These results indicate that the incapability of bacteroids to reestablish growth in nutrient media may not be caused by a decrease in nucleic acid content of the symbiotic rhizobia.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BERGERSEN F. J. The bacterial component of soybean root nodules; changes in respiratory activity, cell dry weight and nucleic acid content with increasing nodule age. J Gen Microbiol. 1958 Oct;19(2):312–323. doi: 10.1099/00221287-19-2-312. [DOI] [PubMed] [Google Scholar]
- Cowles J. R., Evans H. J. Some properties of the ribonucleotide reductase from Rhizobium meliloti. Arch Biochem Biophys. 1968 Sep 20;127(1):770–778. doi: 10.1016/0003-9861(68)90288-9. [DOI] [PubMed] [Google Scholar]
- Crissman H. A., Steinkamp J. A. Rapid, simultaneous measurement of DNA, protein, and cell volume in single cells from large mammalian cell populations. J Cell Biol. 1973 Dec;59(3):766–771. doi: 10.1083/jcb.59.3.766. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JORDAN D. C., GRINYER I., COULTER W. H. ELECTRON MICROSCOPY OF INFECTION THREADS AND BACTERIA IN YOUNG ROOT NODULES OF MEDICAGO SATIVA. J Bacteriol. 1963 Jul;86:125–137. doi: 10.1128/jb.86.1.125-137.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paau A., Cowles J. R. Comparison of DNA Polymerase of Rhizobium meliloti and Alfalfa Bacteroids. Plant Physiol. 1975 Oct;56(4):526–528. doi: 10.1104/pp.56.4.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinkamp J. A., Fulwyler M. J., Coulter J. R., Hiebert R. D., Horney J. L., Mullancy P. F. A new multiparameter separator for microscopic particles and biological cells. Rev Sci Instrum. 1973 Sep;44(9):1301–1310. doi: 10.1063/1.1686375. [DOI] [PubMed] [Google Scholar]
- Sutton W. D. Some features of the DNA of Rhizobium bacteroids and bacteria. Biochim Biophys Acta. 1974 Sep 27;366(1):1–10. doi: 10.1016/0005-2787(74)90312-8. [DOI] [PubMed] [Google Scholar]