Figure 6.
Inhibition of cellular protein tyrosine kinases leads to autonomous replication of the wt AAV genome. Cells were infected with the wt AAV at an moi of 2 following either no treatment or treatment with HU, genistein, or NaOV, and low-Mr DNA samples isolated at various indicated times postinfection were analyzed on Southern blots using an AAV-specific DNA probe. d, m, and ss denote the dimeric and monomeric replicative DNA intermediates and single-stranded progeny AAV genomes, respectively.